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Rat Insulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00515

Novus Biologicals, part of Bio-Techne
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NBP3-00515

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (100 uL)

Sensitivity

3.75 pg/mL

Assay Range

6.25 - 400 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 4.61

Inter-Assay Precision (Precision between assays) %CV < 4.92

Recovery for Rat Insulin ELISA Kit (Colorimetric)

Recovery

83 - 100%

Linearity

Rat Insulin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Insulin ELISA Kit (Colorimetric)

Rat Insulin ELISA Kit (Colorimetric)

ELISA: Rat Insulin ELISA Kit (Colorimetric) [NBP3-00515] -

ELISA: Rat Insulin ELISA Kit (Colorimetric) [NBP3-00515] - Standard Curve Reference

Kit Contents for Rat Insulin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Insulin

Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as a-cell of islets of Langerhans and tumor (insulinoma) marker.

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Rat Insulin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Insulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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