Human IRS1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68045

Novus Biologicals, part of Bio-Techne
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NBP2-68045
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all IRS1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IRS1 in samples. No significant cross-reactivity or interference between Human IRS1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.79%

Inter-Assay Precision (Precision between assays) CV% < 5.29%

Recovery for Human IRS1 ELISA Kit (Colorimetric)

Recovery

84-109%

Linearity

Human IRS1 ELISA Kit (Colorimetric)

Scientific Data Images for Human IRS1 ELISA Kit (Colorimetric)

Human IRS1 ELISA Kit (Colorimetric)

ELISA: Human IRS1 ELISA Kit (Colorimetric) [NBP2-68045] -

ELISA: Human IRS1 ELISA Kit (Colorimetric) [NBP2-68045] - Standard Curve Reference

Kit Contents for Human IRS1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IRS1

Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). IRS-1 functions as one of the key regulators of IR and the insulin-like growth factor-1 receptor. Disruption of IRS-1 causes growth retardation and insulin resistance associated with hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation (4-5)

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Gene Symbol

IRS1

Additional IRS1 Products

Product Documents for Human IRS1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IRS1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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