Mouse Laminin ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # KA0380
Key Product Details
Sample Type & Volume Required Per Well
Cell lysates, Cell Culture Supernatant, Plasma (EDTA, Heparin), Serum (100 uL)
Sensitivity
10 pg/mL
Assay Range
156 to 10000 pg/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Mouse
Description
Quality control test: Standard curve. Laminin (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse laminin.
Scientific Data Images for Mouse Laminin ELISA Kit (Colorimetric)
![ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380]](https://resources.bio-techne.com/images/products/Laminin-ELISA-Kit-[Biotin]-ELISA-KA0380-img0001.jpg "ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380]")
ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380]
ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380] - Standard curve for Mouse Laminin ELISA Kit (Colorimetric) [KA0380].Kit Contents for Mouse Laminin ELISA Kit (Colorimetric)
- 96-well plate precoated with anti- mouse Laminin antibody
- ABC diluent buffer
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
- Biotinylated anti-mouse Laminin antibody, dilution 1:100
- Lyophilized mouse Laminin standard
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Laminin
Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).
References
1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214
2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567
3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y., . . . Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5
Alternate Names
LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha
Gene Symbol
LAMA1
Additional Laminin Products
Product Documents for Mouse Laminin ELISA Kit (Colorimetric)
Product Specific Notices for Mouse Laminin ELISA Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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