Canine Leptin/OB ELISA Kit (Colorimetric)

Name: Canine Leptin/OB ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-42561

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42561

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma and other biological fluids (100 uL)

Sensitivity

0.112 ng/mL

Assay Range

0.312 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Canine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Canine Leptin/OB ELISA Kit (Colorimetric)

Recovery

84 - 97%

Kit Contents for Canine Leptin/OB ELISA Kit (Colorimetric)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Alternate Names

LEP, OB

Gene Symbol

LEP

Additional Leptin/OB Products

Product Documents for Canine Leptin/OB ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Canine Leptin/OB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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