Porcine Leptin/OB ELISA Kit (Chemiluminescence)

Name: Porcine Leptin/OB ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP3-42631

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42631

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

4.9 pg/mL

Assay Range

13.7 - 10000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Porcine

Description

Assay time: 2 hours, 40 minutes

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Porcine Leptin/OB ELISA Kit (Chemiluminescence)

Recovery

88 - 99%

Kit Contents for Porcine Leptin/OB ELISA Kit (Chemiluminescence)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Substrate A
Substrate B
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Alternate Names

LEP, OB

Gene Symbol

LEP

Additional Leptin/OB Products

Product Documents for Porcine Leptin/OB ELISA Kit (Chemiluminescence)

Product Datasheets

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COA

Product Specific Notices for Porcine Leptin/OB ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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