Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83730

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma, urine, synovial fluid, amniotic fluid (100 uL)

Sensitivity

4.6 pg/mL

Assay Range

15.6 - 1,000 pg/mLml

View all Lymphotoxin-alpha/TNF-beta ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF beta without TNF alpha cross reactivity.

Description

The human Lymphotoxin-alpha/TNF-beta ELISA is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.0%

Inter-Assay Precision (Precision between assays) CV% < 10.2%

Recovery for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 87% to 130% with an overall mean recovery of 105%

Scientific Data Images for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

ELISA: Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP1-83730]

ELISA: Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP1-83730]

ELISA: Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP1-83730] - Representative standard curve for human TNF beta ELISA. Human TNF beta was diluted in serial 2-fold steps inSample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Kit Contents for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
  • anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
  • Anti-rabbit-IgG-HRP
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
  • Lysis Buffer 10x
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lymphotoxin-alpha/TNF-beta

Tumor Necrosis Factor (TNF) was originally discovered as a serum protein with necrotizing effects on certain transplantable mouse tumors in vivo and cytotoxic effects against some transformed cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD mainly produced by activated lymphocytes. TNF-beta is 28 % structurally homologous with the macrophage produced non-glycoprotein TNF-alpha.TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo.The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF-beta without TNF-alpha cross reactivity.

Long Name

Tumor Necrosis Factor beta

Alternate Names

LTA, Lymphotoxin alpha, Lymphotoxinalpha, TNF-beta, tnfb, TNFSF1, TNFSF1B

Gene Symbol

LTA

Additional Lymphotoxin-alpha/TNF-beta Products

Product Documents for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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