Chicken Lysozyme ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60088

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and egg white (50 - 100 uL)

Sensitivity

14.876 ng/mL

Assay Range

15.625 ng/mL - 4000 ng/mL

View all Lysozyme ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Chicken

Specificity

This assay has high sensitivity and excellent specificity for detection of chicken lysozyme. No significant cross-reactivity or interference between chicken lysozyme and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Chicken Lysozyme ELISA Kit (Colorimetric)

ELISA: Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088]

ELISA: Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088]

ELISA: Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Chicken Lysozyme ELISA Kit (Colorimetric)

Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088] -

Chicken Lysozyme ELISA Kit (Colorimetric) [NBP2-60088] - Standard Curve Reference

Kit Contents for Chicken Lysozyme ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • Biotin-conjugate (100 x concentrate)
  • Biotin-conjugate Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lysozyme

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for lysozyme has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated lysozyme. A competitive inhibition reaction is launched between lysozyme (Standards or samples) and biotin-conjugated lysozyme with the pre-coated lysozyme antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of lysozyme bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

Gene Symbol

LYZ

Additional Lysozyme Products

Product Documents for Chicken Lysozyme ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Chicken Lysozyme ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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