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Human M-CSF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0384

Novus Biologicals, part of Bio-Techne
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KA0384

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma (EDTA, Heparin), Saliva, Serum, Tissue Homogenates and Urine (100 uL)

Sensitivity

1 pg/mL

Assay Range

62.5 to 4000 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

CSF1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human CSF1.

Kit Contents for Human M-CSF ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti-human M-CSF antibody
  • ABC diluent buffer
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  • Biotinylated anti-human M-CSF antibody, dilution 1:100
  • Lyophilized recombinant human M-CSF standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: M-CSF

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M-CSF ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human M-CSF ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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