Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric)

Name: Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39842

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39842

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Product Datasheet / COA

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.117 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric)

ELISA: Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric) [NBP3-39842]

ELISA: Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric) [NBP3-39842] - Standard Curve Reference

Kit Contents for Human Meprin beta Subunit/MEP1B ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Meprin beta Subunit/MEP1B

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells. Mature meprins are oligomers of evolutionarily related, separately encoded alpha and/or beta subunits. Homooligomers of meprin-alpha are secreted; oligomers containing meprin-beta are associated with the plasma membrane. Substrates include bioactive peptides and extracellular matrix proteins.

Alternate Names

MEP1B

Gene Symbol