Rat MMP-12 ELISA Kit (Colorimetric)

Name: Rat MMP-12 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06764

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06764

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat MMP-12 in samples. No significant cross-reactivity or interference between Rat MMP-12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.25

Inter-Assay Precision (Precision between assays) 4.88

Recovery for Rat MMP-12 ELISA Kit (Colorimetric)

Recovery

90 - 110 %

Linearity

Scientific Data Images for Rat MMP-12 ELISA Kit (Colorimetric)

ELISA: Rat MMP-12 ELISA Kit (Colorimetric) [NBP3-06764] -

ELISA: Rat MMP-12 ELISA Kit (Colorimetric) [NBP3-06764] - Standard Curve Reference

Kit Contents for Rat MMP-12 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-12

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol