I have some questions about MMP-2 kit KA0391 and TMP-2 kit KA0549. What are they measuring? The active form the pro-form and/or the complex. Can I use EDTA-plasma?

Name: Human MMP-2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA0391

Both of these kits are produced by a Taiwanese company called Abnova and we distribute for them. KA0391 detects both the zymogen and active enzyme and is suitable for body fluid, cell culture supernatant, plasma, serum and tissue lysate samples. KA0549 can detect TIMP2 sera, plasma, body fluids, tissue lysates or cell culture supernatants and Abnova does not distinguish between detecting different forms, so it will probably work for all.

Human MMP-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0391

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Product Specifications
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma (Heparin), Serum (100 uL)

Sensitivity

<10 pg/mL

Assay Range

625 to 40000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

MMP2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human MMP2.

Kit Contents for Human MMP-2 ELISA Kit (Colorimetric)

96-well plate precoated with anti- human MMP-2 antibody.
ABC diluent buffer
Antibody diluent buffer
Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100.
Biotinylated anti- human MMP-2 antibody, dilution 1:100.
Lyophilized recombinant human MMP-2 standard
Sample diluent buffer
TMB color developing agent
TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. MMP2 triggers cells to begin migration by cleaving laminin-5 and exposing an integrin-binding site on the epithelial basement membrane. The cleaved of laminin has been detected in tumors and in tissues being altered, howver the activated form of MMP2 is not found in benign tumors. Thus, detection of MMP2 is a possible early indicator of tumor activity. MMP2 is most strongly expressed in normal skin fibroblasts, but may also be found in gliomas, breast and prostate tumors. Mutations in the MMP-2 gene have been linked to Torg-Winchester syndrome, Nodulosis-Arthropathy-Osteolysis (NAO) syndrome, and arthritis. Additionally, abnormal expression of matrix matalloproteinases have been associated with neoplastic traits such as loss of negative growth regulation and high invasive potential.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Human MMP-2 ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download MMP2 (Human) ELISA Kit

COA

Product Specific Notices for Human MMP-2 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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