Rat MMP-3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06894

Novus Biologicals, part of Bio-Techne
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NBP3-06894
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all MMP-3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.29

Inter-Assay Precision (Precision between assays) 4.95

Recovery for Rat MMP-3 ELISA Kit (Colorimetric)

Recovery

85-110 %

Linearity

Rat MMP-3 ELISA Kit (Colorimetric)

Scientific Data Images for Rat MMP-3 ELISA Kit (Colorimetric)

Rat MMP-3 ELISA Kit (Colorimetric)

ELISA: Rat MMP-3 ELISA Kit (Colorimetric) [NBP3-06894] -

ELISA: Rat MMP-3 ELISA Kit (Colorimetric) [NBP3-06894] - Standard Curve Reference

Kit Contents for Rat MMP-3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-3

Effective inhibitors of matrix metalloproteinases (MMPs), a family of connective tissue-degrading enzymes, could be useful for the treatment of diseases such as cancer, multiple sclerosis, and arthritis (1). Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. MMP3 (stromelysin) is a secreted metalloprotease with a wide range of substrate specificities. MMP3 is synthesized in a preproenzyme form with a calculated size of 54 kDa and a 17-amino acid long signal peptide. Data indicate that the expression and the possible involvement of secreted metalloproteases in tumorigenesis result from a specific interaction between the transforming factor and the target cell, which may vary in different species (2). In neural studies, matrix metalloproteinase-3 (MMP-3) was newly induced and activated in stressed dopamine cells, and the active form of MMP-3 (actMMP-3) was released into the medium. The released actMMP-3, as well as catalytically active recombinant MMP-3 (cMMP-3) led to microglial activation and superoxide generation in microglia and enhanced DA cell death. (3).

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Gene Symbol

MMP3

Additional MMP-3 Products

Product Documents for Rat MMP-3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat MMP-3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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