Mouse C1qR1/CD93 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCD930
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
11.4 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse C1qR1/CD93 Quantikine ELISA Kit
The Quantikine Mouse C1q R1/CD93 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse C1q R1 levels in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse C1q R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse C1q R1 accurately. Results obtained using natural mouse C1q R1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse C1q R1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse C1q R1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 22 | 22 |
| Mean (pg/mL) | 248 | 704 | 2033 | 256 | 692 | 2085 |
| Standard Deviation | 2.81 | 13.2 | 29.2 | 19.2 | 33.2 | 116 |
| CV% | 1.1 | 1.9 | 1.4 | 7.5 | 4.8 | 5.6 |
Recovery for Mouse C1qR1/CD93 Quantikine ELISA Kit
The recovery of mouse C1q R1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=8) | 95 | 84-105 |
| Cell Lysates (n=4) | 110 | 102-116 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse C1q R1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse C1qR1/CD93 Quantikine ELISA Kit
Mouse C1q R1/CD93 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C1qR1/CD93
Long Name
Complement Component C1q Receptor
Alternate Names
AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68
Gene Symbol
CD93
Additional C1qR1/CD93 Products
Product Documents for Mouse C1qR1/CD93 Quantikine ELISA Kit
Product Specific Notices for Mouse C1qR1/CD93 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.