Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCX120
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (50 µL)
Sensitivity
0.069 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse SDF-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.09 | 2.60 | 4.10 | 1.25 | 3.15 | 4.91 |
| Standard Deviation | 0.04 | 0.12 | 0.21 | 0.09 | 0.24 | 0.37 |
| CV% | 3.7 | 4.6 | 5.1 | 7.2 | 7.6 | 7.5 |
Recovery for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 98-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
Mouse CXCL12/SDF-1 alpha ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL12/SDF-1 alpha
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
Additional CXCL12/SDF-1 alpha Products
Product Documents for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
Product Specific Notices for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.