Mouse CXCL2/MIP-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MM200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
1.5 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
The Quantikine Mouse MIP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MIP-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse MIP-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse MIP-2 accurately. Results obtained using natural mouse MIP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MIP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MIP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 17.7 | 53.9 | 363 | 16.9 | 52.1 | 346 |
| Standard Deviation | 0.5 | 1.3 | 7.3 | 1.0 | 2.3 | 15.4 |
| CV% | 2.8 | 2.4 | 2.0 | 5.9 | 4.4 | 4.5 |
Recovery for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
The recovery of mouse MIP-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=10) | 96 | 84-110 |
| Serum (n=14) | 100 | 94-116 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse MIP-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
Mouse CXCL2/GRO beta/MIP-2/CINC-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
Product Specific Notices for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.