Mouse Fetuin A/AHSG Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MFTA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.337 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Fetuin A/AHSG Quantikine ELISA Kit
The Quantikine Mouse Fetuin A Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Fetuin A levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Fetuin A and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Fetuin A accurately. Results obtained using natural mouse Fetuin A showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fetuin A.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Fetuin A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 6.4 | 16.1 | 47.0 | 6.0 | 16.0 | 47.0 |
| Standard Deviation | 0.260 | 0.561 | 1.16 | 0.446 | 0.722 | 2.71 |
| CV% | 4.1 | 3.5 | 2.5 | 7.4 | 4.5 | 5.8 |
Recovery for Mouse Fetuin A/AHSG Quantikine ELISA Kit
The recovery of mouse Fetuin A spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 100-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Fetuin A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse Fetuin A/AHSG Quantikine ELISA Kit
Mouse Fetuin A/AHSG ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fetuin A/AHSG
Long Name
alpha-2-HS Glycoprotein
Alternate Names
AHSG, alpha-2-HS-glycoprotein
Gene Symbol
AHSG
Additional Fetuin A/AHSG Products
Product Documents for Mouse Fetuin A/AHSG Quantikine ELISA Kit
Product Specific Notices for Mouse Fetuin A/AHSG Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.