Mouse LIX Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MX000
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (13 µL), Platelet-poor Heparin Plasma (13 µL), Urine (13 µL)
Sensitivity
1.74 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Product Summary for Mouse LIX Quantikine ELISA Kit
The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains E. coli-expressed recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse LIX
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 42.7 | 133 | 407 | 41.8 | 120 | 393 |
| Standard Deviation | 1.72 | 5.93 | 10.9 | 2.81 | 4.64 | 20.3 |
| CV% | 4.0 | 4.5 | 2.7 | 6.7 | 3.9 | 5.2 |
Recovery for Mouse LIX Quantikine ELISA Kit
The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 114 | 105-120 |
| Platelet-poor EDTA Plasma (n=4) | 100 | 92-109 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 85-99 |
| Serum (n=4) | 87 | 82-94 |
| Tissue Homogenates (n=4) | 100 | 87-113 |
| Urine (n=4) | 91 | 84-96 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse LIX Quantikine ELISA Kit
Mouse LIX ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIX
Additional LIX Products
Product Documents for Mouse LIX Quantikine ELISA Kit
Product Specific Notices for Mouse LIX Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.