Mouse Progranulin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MPGRN0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.071 ng/mL
Assay Range
0.625-20 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Progranulin Quantikine ELISA Kit
The Quantikine® Mouse Progranulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Progranulin in cell culture supernates, tissue culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Progranulin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Progranulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.75 | 2.94 | 7.28 | 1.69 | 2.89 | 7.53 |
| Standard Deviation | 0.050 | 0.067 | 0.160 | 0.092 | 0.137 | 0.336 |
| CV% | 2.9 | 2.3 | 2.2 | 5.4 | 4.7 | 4.5 |
Recovery for Mouse Progranulin Quantikine ELISA Kit
The recovery of mouse Progranulin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Culture Media (n=4) | 98 | 94-104 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Progranulin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse Progranulin Quantikine ELISA Kit
Mouse Progranulin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Progranulin/PGRN
Alternate Names
Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin
Gene Symbol
GRN
Additional Progranulin/PGRN Products
Product Documents for Mouse Progranulin Quantikine ELISA Kit
Product Specific Notices for Mouse Progranulin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.