Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MB200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Cell Lysates (25 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
4.26 pg/mL
Assay Range
12.5-800 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat, Porcine, Canine
Specificity
Recombinant and natural mouse, rat, canine and porcine TGF-beta 2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 29.0 | 102 | 323 | 29.9 | 115 | 337 |
| Standard Deviation | 2.43 | 4.97 | 21.0 | 2.80 | 8.20 | 30.7 |
| CV% | 8.4 | 4.9 | 6.5 | 9.4 | 7.2 | 9.1 |
Recovery for Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
The recovery of TGF-beta 2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 92 | 80-102 |
| Cell Lysates (n=2) | 94 | 86-98 |
| EDTA Plasma (n=4) | 84 | 78-94 |
| Heparin Plasma (n=4) | 82 | 74-92 |
| Serum (n=4) | 98 | 85-114 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of TGF-beta 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
Mouse/Rat/Porcine/Canine TGF-beta 2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TGF-beta 2
TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also
known as TGF-beta RIII) and the TGF-beta RII type II ser/thr kinase receptor (15). In contrast, TGF-beta 1
and 3 have higher affinity for TGF-beta RII and do not require betaglycan (15). TGF-beta RII then
phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin
receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and
activates Smad proteins that regulate transcription (1, 6, 15, 16). Differences in structure of the
prodomains and mature sequences of TGF-beta isoforms, and use of Smad-independent signaling
pathways, allow for disparate actions observed in response to different TGF-beta isoforms and
contexts (1-3, 12, 13, 15, 16).
Although many functions are overlapping, each TGF-beta isoform has some non-redundant
functions. TGF-beta 2 plays a non-redundant role in human and mouse developmental heart valve
remodeling, and mice with targeted deletions of TGF-beta 2 show defects in development of the
cardiac system as well as lung, craniofacial, limb, eye, ear and urogenital systems (2, 7, 17, 28).
TGF-beta 2 plays a unique positive regulatory role in hematopoiesis by enhancing Flt-3 signaling in
hematopoietic progenitors (19). In humans, TGF-beta isoforms, especially TGF-beta 2, are identified as
key factors in the progression of malignant glioma, gastric and ovarian cancer (8, 20-22).
TGF-beta isoforms, particularly TGF-beta 2, suppress macrophage cytokine production and mucosal
inflammatory responses in the developing intestine (23). In turn, macrophage LRP-1 can
downregulate expression of TGF-beta 2 during vascular remodeling, which suppresses neo-intima
formation (24).
Long Name
Transforming Growth Factor beta 2
Alternate Names
TGFB2, TGFbeta 2
Gene Symbol
TGFB2
Additional TGF-beta 2 Products
Product Documents for Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.