Mouse/Rat Cystatin C Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MSCTC0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
12.9 pg/mL
Assay Range
125-8000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse/Rat Cystatin C Quantikine ELISA Kit
The Quantikine Mouse/Rat Cystatin C Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Cystatin C in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Cystatin C and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse or rat Cystatin C showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat Cystatin C.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat Cystatin C
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 434 | 894 | 3065 | 465 | 942 | 3257 |
| Standard Deviation | 14.9 | 29.2 | 85.9 | 43.5 | 51.2 | 213 |
| CV% | 3.4 | 3.3 | 2.8 | 9.4 | 5.4 | 6.5 |
Recovery for Mouse/Rat Cystatin C Quantikine ELISA Kit
The recovery of Cystatin C spiked into cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 98-113 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse or rat Cystatin C were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat Cystatin C Quantikine ELISA Kit
Mouse/Rat Cystatin C ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cystatin C
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
Additional Cystatin C Products
Product Documents for Mouse/Rat Cystatin C Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat Cystatin C Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.