Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MFB00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (10 µL), Serum (50 µL), EDTA Plasma (50 µL)
Sensitivity
3.68 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma)
Product Summary for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E.
coli-expressed
recombinant rat FGF basic and antibodies raised against the recombinant
factor. This immunoassay has been shown to quantitate the recombinant
factor accurately. Results obtained using natural FGF basic showed
dose-response curves that were parallel to the standard curves obtained
using the recombinant kit standards. These results indicate that this
kit can be used to determine relative mass values for natural FGF basic.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant FGF basic. This assay cross-reacts 100% with Bovine FGF basic, recombinant Human FGF basic is detectable.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 49.5 | 122 | 394 | 45.0 | 104 | 382 |
| Standard Deviation | 1.17 | 2.7 | 12.0 | 3.80 | 5.03 | 20.5 |
| CV% | 2.4 | 2.2 | 3.1 | 8.4 | 4.8 | 5.4 |
Recovery for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 101 | 91-110 |
| EDTA Plasma (n=4) | 97 | 84-110 |
| Serum (n=4) | 95 | 84-110 |
| Tissue Lysates (n=4) | 97 | 80-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FGF basic were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
Mouse/Rat FGF basic ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF basic/FGF2/bFGF
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
Additional FGF basic/FGF2/bFGF Products
Product Documents for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat FGF basic/FGF2/bFGF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.