Mouse/Rat GDF-15 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MGD150
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
2.2 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse/Rat GDF-15 Quantikine ELISA Kit
The Quantikine Mouse/Rat GDF-15 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat GDF-15 in cell culture supernates, serum, plasma, and urine. It contains E.
coli-expressed recombinant mouse GDF-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat GDF-15.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural mouse and rat GDF-15, recombinant mouse mature GDF-15, precursor GDF-15, and GDF-15/BMP-2 heterodimer. It does not detect monomeric recombinant mouse GDF-15.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples were evaluated for the presence of mouse/rat GDF-15 in this assay.
ND=Non-detectable
| Mouse Samples | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=10) | 77.0 | 47.4-97.1 | 19.5 |
| EDTA plasma (n=5) | 241 | 54.4-874 | 354 |
| Heparin plasma (n=5) | 110 | 82.4-169 | 34.7 |
| Urine (n=10) | 2376 | 632-6255 | 1547 |
| Rat Samples | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=10) | 27.5 | 10.5-49.4 | 14.3 |
| Heparin plasma (n=5) | 42.7 | 11.1-145 | 57.3 |
| Urine (n=10) | 4972 | 1922-11,550 | 2747 |
| Rat Samples | Mean of Detectable (pg/mL) | % Detectable | Standard Deviation (pg/mL) |
| EDTA plasma (n=5) | 31.5 | 80 | ND-68.8 |
Cell Culture Supernates:
C2C12 mouse myoblast cells were cultured in DMEM supplemented with 10% fetal bovine
serum to 80% confluency. Cell were washed with PBS and cultured in DMEM supplemented
with 5% equine serum for 6 days with media changes every other day. When the cells were
50% differentiated, they were unstimulated or stimulated with 1000 μM H2O2 for 1 day. Aliquots
of the cell culture supernates were removed, assayed for mouse/rat GDF-15, and measured
4630 pg/mL and 7450 pg/mL, respectively.
Hepa 1-6 mouse hepatoma cells (1 x 106
cells/mL) were cultured for 6 days in DMEM
supplemented with 10% fetal bovine serum and 2 mM L-glutamine. An aliquot of the cell
culture supernate was removed, assayed for mouse/rat GDF-15, and measured 6255 pg/mL.
H4-ll-E-C3 rat hepatoma cells (2 x 106
cells/mL) were cultured for 3 days in DMEM
supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL of streptomycin sulfate. An aliquot of the cell culture supernate was removed,
assayed for mouse/rat GDF-15, and measured 514 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 25.6 | 60.0 | 190 | 26.7 | 63.1 | 196 |
| Standard Deviation | 0.690 | 1.42 | 5.49 | 1.62 | 2.84 | 10.7 |
| CV% | 2.7 | 2.4 | 2.9 | 6.1 | 4.5 | 5.5 |
Recovery for Mouse/Rat GDF-15 Quantikine ELISA Kit
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Media (n=4) | 102 | 91-110 |
| Mouse EDTA Plasma (n=4) | 95 | 81-109 |
| Mouse Heparin Plasma (n=4) | 95 | 87-104 |
| Mouse Serum (n=4) | 91 | 82-101 |
| Mouse Urine (n=4) | 97 | 88-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat GDF-15 Quantikine ELISA Kit
Mouse/Rat GDF-15 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-15
Long Name
Growth Differentiation Factor 15
Alternate Names
GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta
Gene Symbol
GDF15
Additional GDF-15 Products
Product Documents for Mouse/Rat GDF-15 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat GDF-15 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.