Mouse/Rat IL-17F Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M17F0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
1.74 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat IL-17F Quantikine ELISA Kit
The Quantikine Mouse/Rat IL-17F Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-17F in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17F and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-17F showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-17F.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat IL-17F.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 56.7 | 211 | 409 | 53.2 | 202 | 399 |
| Standard Deviation | 1.26 | 4.93 | 13.1 | 3.98 | 11.4 | 20.2 |
| CV% | 2.2 | 2.3 | 3.2 | 7.5 | 5.6 | 5.1 |
Recovery for Mouse/Rat IL-17F Quantikine ELISA Kit
The recovery of mouse IL-17F spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 92-99 |
| EDTA Plasma (n=4) | 107 | 101-118 |
| Heparin Plasma (n=4) | 101 | 88-121 |
| Serum (n=4) | 95 | 83-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17F in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat IL-17F Quantikine ELISA Kit
Mouse/Rat IL-17F ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17F
Long Name
Interleukin 17F
Alternate Names
IL17F
Gene Symbol
IL17F
Additional IL-17F Products
Product Documents for Mouse/Rat IL-17F Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat IL-17F Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.