Mouse/Rat IL-33 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M3300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
14.3 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat IL-33 Quantikine ELISA Kit
The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natural IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat IL-33
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 41 | 41 | 41 |
| Mean (pg/mL) | 58.2 | 298 | 630 | 57.6 | 282 | 599 |
| Standard Deviation | 5.6 | 17.4 | 33.5 | 5.89 | 16.3 | 30.0 |
| CV% | 9.6 | 5.8 | 5.3 | 10.2 | 5.8 | 5.0 |
Recovery for Mouse/Rat IL-33 Quantikine ELISA Kit
The recovery of IL-33 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=4) | 104 | 91-116 |
| Mouse EDTA Plasma (n=4) | 102 | 96-113 |
| Mouse Heparin Plasma (n=4) | 99 | 83-111 |
| Mouse Serum (n=4) | 90 | 81-103 |
| Rat Cell Culture Supernates (n=4) | 104 | 94-116 |
| Rat EDTA Plasma (n=4) | 93 | 83-100 |
| Rat Heparin Plasma (n=4) | 93 | 87-99 |
| Rat Serum (n=4) | 93 | 86-101 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-33 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat IL-33 Quantikine ELISA Kit
Mouse/Rat IL-33 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-33
Long Name
Interleukin 33
Alternate Names
C9orf26, DVS27, IL33, NF-HEV
Gene Symbol
IL33
Additional IL-33 Products
Product Documents for Mouse/Rat IL-33 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat IL-33 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.