Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MSST00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.17 pg/mL
Assay Range
15.6-1000 pg/mL
Product Summary for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
The Quantikine Mouse/Rat SOST immunoassay is a 4.5 hour solid-phase ELISA designed to measure SOST in mouse or rat cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse SOST and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SOST. Results obtained using natural mouse or rat SOST showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SOST.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat SOST
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 57.6 | 108 | 345 | 54.4 | 101 | 347 |
| Standard Deviation | 4.15 | 6.00 | 18.7 | 4.35 | 6.23 | 19.4 |
| CV% | 7.2 | 5.6 | 5.4 | 8.0 | 6.2 | 5.6 |
Recovery for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
The recovery of SOST spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 101 | 92-112 |
| Mouse EDTA Plasma (n=4) | 98 | 82-113 |
| Mouse Heparin Plasma (n=4) | 102 | 88-115 |
| Mouse Serum (n=4) | 95 | 86-113 |
| Rat EDTA Plasma (n=2) | 107 | 101-118 |
| Rat Heparin Plasma (n=2) | 96 | 82-111 |
| Rat Serum (n=4) | 99 | 85-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SOST in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
Mouse/Rat SOST/Sclerostin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SOST/Sclerostin
Alternate Names
sclerostin, VBCHsclerosteosis
Gene Symbol
SOST
Additional SOST/Sclerostin Products
Product Documents for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.