Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt

Name: Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt
Brand: R&D Systems
SKU: DYC897-2

R&D Systems, part of Bio-Techne | Catalog # DYC897-2

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Citations (1)

Product Datasheet / COA / SDS

New Version Available - MCTC0

Discontinued Product

DYC897-2 has been discontinued. An alternative/replacement product is available: MCTC0. View all Cytochrome c products.

Product Summary
Product Specifications
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Sample Type

Cell Lysates

Reactivity

Mouse, Rat

Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

View all Cytochrome c ELISA Kits »

Product Summary for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Cytochrome c in cell lysates. An immobilized capture antibody specific for Cytochrome c binds both phosphorylated and unphosphorylated Cytochrome c. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Mouse/Rat Total Cytochrome c DuoSet IC, Short Format, 2 Plt

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytochrome c

Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol