Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MTHBD0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
19.1 pg/mL
Assay Range
46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
The Quantikine Mouse Thrombomodulin/BDCA-3 immunoassay is a 4.5 hour solid-phase ELISA designed to measure Thrombomodulin in mouse cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Thrombomodulin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Thrombomodulin. Results obtained using natural mouse Thrombomodulin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Thrombomodulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Thrombomodulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 108 | 388 | 1159 | 121 | 380 | 1155 |
| Standard Deviation | 6.67 | 16.0 | 44.8 | 16.0 | 38.1 | 136 |
| CV% | 6.2 | 4.1 | 3.9 | 13.2 | 10.0 | 11.8 |
Recovery for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
The recovery of mouse Thrombomodulin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 91-115 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Thrombomodulin in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. All samples were diluted prior to assay.
Scientific Data Images for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
Mouse Thrombomodulin/BDCA-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thrombomodulin/BDCA-3
Alternate Names
BDCA-3, BDCA3, CD141, Fetomodulin, THBD, THRM
Gene Symbol
THBD
Additional Thrombomodulin/BDCA-3 Products
Product Documents for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
Product Specific Notices for Mouse Thrombomodulin/BDCA-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.