Mouse TWEAK/TNFSF12 DuoSet ELISA

Name: Mouse TWEAK/TNFSF12 DuoSet ELISA
Brand: R&D Systems
SKU: DY1237

R&D Systems, part of Bio-Techne | Catalog # DY1237

Submit a review

Citations (5)

Product Datasheet / COA / SDS

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse TWEAK/TNFSF12 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all TWEAK/TNFSF12 ELISA Kits »

Product Summary for Mouse TWEAK/TNFSF12 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse TWEAK / TNFSF12. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse TWEAK/TNFSF12 DuoSet ELISA

Mouse TWEAK / TNFSF12 ELISA Standard Curve

Kit Contents for Mouse TWEAK/TNFSF12 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily. The extracellular domains of human and mouse TWEAK share 89% amino acid sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecule by proteolytic cleavage after Arg 93, suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Entrez Gene IDs

8742 (Human); 21944 (Mouse); 360548 (Rat)

Gene Symbol