Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81181

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all NCAM-1/CD56 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat NCAM-1/CD56 in samples. No significant cross-reactivity or interference between Rat NCAM-1/CD56 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.43%

Inter-Assay Precision (Precision between assays) CV% < 5.18%

Recovery for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

Recovery

88-107%

Linearity

Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

Scientific Data Images for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

ELISA: Rat NCAM-1/CD56 ELISA Kit (Colorimetric) [NBP2-81181] -

ELISA: Rat NCAM-1/CD56 ELISA Kit (Colorimetric) [NBP2-81181] - Standard Curve Reference

Kit Contents for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

Additional NCAM-1/CD56 Products

Product Documents for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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