Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81182

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all Neutrophil Elastase/ELA2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Neutrophil Elastase/ELA2 in samples. No significant cross-reactivity or interference between Rat Neutrophil Elastase/ELA2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.49%

Inter-Assay Precision (Precision between assays) CV% < 4.18%

Recovery for Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

ELISA: Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric) [NBP2-81182] -

ELISA: Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric) [NBP2-81182] - Standard Curve Reference

Kit Contents for Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Neutrophil Elastase/ELA2

The ELANE gene (commonly known as neutrophil elastase) encodes a 267 amino acid long, 28 kDA neutrophil elastase protein that functions in regulation of natural killer cells, monocytes, and graulocytes. ELANE is involved in activation of proMMP8, cell adhesion cell-matrix glycoconjugates, selected targets of C/EBPbeta, degradation of the extracellular matrix and collagen, and amb2 integrin signaling. It interacts with various genes GZMB, LRP1, SERPINF2, SERPING1, and SERPINA1. Elane has been linked to cystic fibrosis, asthma, pneumonia, alzheimer's disease, neutropenia, cutis laxia, leg ulcers, wegener's granulomatosis, bronchitis, adult respiratory distress syndrome, and vascular diseases.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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