Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78763
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
46.88 pg/mL
Assay Range
78.13 - 5000 pg/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed.
Description
This ELISA kit applies to the in vitro quantitative determination of Human pNF-H concentrations in Serum, plasma and other biological fluids.
NFH is phosphorylated on a number of the serines at C-terminal according to the sequence of P12036 in Uniprot.
NFH is phosphorylated on a number of the serines at C-terminal according to the sequence of P12036 in Uniprot.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 5.26%
Inter-Assay Precision (Precision between assays) CV% < 5.01%
Recovery for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
Recovery
86-110%
Linearity
Scientific Data Images for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
ELISA: Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric) [NBP2-78763] -
ELISA: Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric) [NBP2-78763] - Standard Curve ReferenceKit Contents for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: NF-H
Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Gene Symbol
NEFH
Additional NF-H Products
Product Documents for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
Product Specific Notices for Human NF-H [p Ser668, p Ser702] ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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