Mouse Nrf2 ELISA Kit (Colorimetric)

Name: Mouse Nrf2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-76758

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76758

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Product Datasheet / COA / SDS / Protocols & Manuals

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.188ng/mL

Assay Range

0.313 - 20ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.13

Inter-Assay Precision (Precision between assays) CV% < 5.39

Recovery for Mouse Nrf2 ELISA Kit (Colorimetric)

Recovery

86-107%

Linearity

Scientific Data Images for Mouse Nrf2 ELISA Kit (Colorimetric)

ELISA: Mouse Nrf2 ELISA Kit (Colorimetric) [NBP2-76758] -

ELISA: Mouse Nrf2 ELISA Kit (Colorimetric) [NBP2-76758] - Standard Curve Reference

Kit Contents for Mouse Nrf2 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol