Rat Nrf2 ELISA Kit (Colorimetric)

Name: Rat Nrf2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-08161

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08161

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Product Datasheet / COA / SDS / Protocols & Manuals

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.08

Inter-Assay Precision (Precision between assays) %CV < 5.99

Recovery for Rat Nrf2 ELISA Kit (Colorimetric)

Recovery

87 - 104%

Linearity

Scientific Data Images for Rat Nrf2 ELISA Kit (Colorimetric)

ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] -

ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Standard Curve Reference

Kit Contents for Rat Nrf2 ELISA Kit (Colorimetric)

Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol