Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)

Name: Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-42761

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42761

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates and other biological fluids (100 uL)

Sensitivity

1.02 pg/mL

Assay Range

3.12 - 200 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Canine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)

Recovery

79 - 99%

Kit Contents for Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Canine Osteopontin/OPN - High Sensitivity ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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