Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

Name: Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-60100

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60100

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

3.9 pg/mL

Assay Range

15.6 pg/mL - 1000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse OPG. No significant cross-reactivity or interference between mouse OPG and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

ELISA: Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60100]

ELISA: Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60100] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60100] -

Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60100] - Standard Curve Reference

Kit Contents for Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Assay plate (12 x 8 coated Microwells)
Biotin-antibody (100 x concentrate)
Biotin-antibody Diluent
HRP-avidin (100 x concentrate)
HRP-avidin Diluent
Instruction manual
Sample Diluent
Standard (Freeze dried)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Osteoprotegerin/TNFRSF11B

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

OCIF, TNFRSF11B

Gene Symbol

TNFRSF11B

Additional Osteoprotegerin/TNFRSF11B Products

Product Documents for Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download NBP2-60100 ELISA Kit

COA

SDS

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Product Specific Notices for Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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