Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22043
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum (25 ul)
Sensitivity
1.8 pg/mL
Assay Range
78 - 5,000 pg/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The assay detects both natural and recombinant human OX40/TNFRSF4. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human OX40/TNFRSF4 positive serum. There was no crossreactivity detected.
Description
The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 8.0%
Inter-Assay Precision (Precision between assays) CV% < 12.0%
Recovery for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
Recovery
88%
Scientific Data Images for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
ELISA: Human OX40/TNFRSF4 ELISA Kit (Colorimetric) [NBP2-22043]
Kit Contents for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD134/OX40
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
- Blue-Dye
- Green-Dye
- Human sCD134/OX40 Standard lyophilized, 10 ng/ml upon reconstitution
- Red-Dye
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: OX40/TNFRSF4
OX40 (CD134) is a member of the tumor necrosis (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T-cells. Interaction of OX40 with its ligand, OX40L, is thought to be important in T cell activation through T cell/antigen-presenting cell (APC) interaction. Ligation of OX40 induces clonal expansion and survival of CD4 cells during primary responses, and results in the accumulation of greater numbers of memory cells with time.
A soluble isoform of OX40 has been described. Measurement of this molecule may have diagnostic value in polymyositis and granulomatous myopathy, in T cell lymphoma and lymphomatoid papulosis, in proliferative lupus nephritis, in rheumatoid arthritis, in HIV infection, in viral infections of the lung, in the regulation of graft-versus-host disease,in myocarditis and dilated cardiomyopathy.
Alternate Names
ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, tax-transcriptionally activated glycoprotein 1 receptor, TNFRSF4, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4, Txgp1, TXGP1L, TXGP1LOX40 antigen
Gene Symbol
TNFRSF4
Additional OX40/TNFRSF4 Products
Product Documents for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
Product Specific Notices for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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