Monkey P-Selectin/CD62P ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92719

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (baboon) (10 uL)

Sensitivity

1.3 U/mL

Assay Range

0.8 - 50 U/mL

View all P-Selectin/CD62P ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a monkey P-Selectin/CD62P positive serum. There was no crossreactivity detected.

Description

The monkey sP-selectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sP-selectin.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10%

Inter-Assay Precision (Precision between assays) CV% < 10%

Scientific Data Images for Monkey P-Selectin/CD62P ELISA Kit (Colorimetric)

ELISA: Primate P-Selectin/CD62P ELISA Kit (Colorimetric) [NBP1-92719]

Kit Contents for Monkey P-Selectin/CD62P ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sP-selectin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • HRP-Conjugate anti-monkey sP-selectin monoclonal antibody
  • Monkey sP-selectin Standard lyophilized, 100.0 U/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: P-Selectin/CD62P

P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA derived amino acid sequence predicts a molecule with a series of cysteine-rich domains. P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes. The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain. Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemia-reperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for Monkey P-Selectin/CD62P ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Monkey P-Selectin/CD62P ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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