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Human p27/Kip1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66683

Novus Biologicals, part of Bio-Techne
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NBP2-66683

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human P27 in samples. No significant cross-reactivity or interference between human P27 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.09%

Inter-Assay Precision (Precision between assays) CV% < 5.1%

Recovery for Human p27/Kip1 ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Human p27/Kip1 ELISA Kit (Colorimetric)

Scientific Data Images for Human p27/Kip1 ELISA Kit (Colorimetric)

Human p27/Kip1 ELISA Kit (Colorimetric)

ELISA: Human p27/Kip1 ELISA Kit (Colorimetric) [NBP2-66683] -

ELISA: Human p27/Kip1 ELISA Kit (Colorimetric) [NBP2-66683] - Standard Curve Reference

Kit Contents for Human p27/Kip1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for Human p27/Kip1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human p27/Kip1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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