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Human p53 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91257

Novus Biologicals, part of Bio-Techne
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NBP1-91257

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

0.33 U/mL

Assay Range

0.78 - 50 U/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The human p53 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human p53.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.50%

Inter-Assay Precision (Precision between assays) CV% < 8.90%

Recovery for Human p53 ELISA Kit (Colorimetric)

Recovery

95%

Scientific Data Images for Human p53 ELISA Kit (Colorimetric)

Human p53 ELISA Kit [NBP1-91257] - Data for Human p53 Platinum ELISA

Kit Contents for Human p53 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human p53
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human p53 monoclonal antibody
  • Human p53 Standard lyophilized, 100 U/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Formulation

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p53

p53 is a tumor suppressor that is regulated by transcriptional, post-transcriptional, and post-translational mechanisms in response to stress. Studies demonstrate that p53 is critical for the prevention of tumorigenesis and may potentially be involved in at least half of all known cancers. p53 functions as a transcription factor to regulate genes involved in apoptosis, cell cycle, senescence, metabolism, angiogenesis, immunity, differentiation, and migration. Moreover, p53 is phosphorylated at multiple sites, including Ser15, following DNA damage.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for Human p53 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human p53 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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