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Human PAR2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06966

Novus Biologicals, part of Bio-Techne
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NBP3-06966

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human PAR2 in samples. No significant cross-reactivity or interference between Human PAR2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.65

Inter-Assay Precision (Precision between assays) %CV < 5.01

Recovery for Human PAR2 ELISA Kit (Colorimetric)

Recovery

86 - 107%

Linearity

Human PAR2 ELISA Kit (Colorimetric)

Scientific Data Images for Human PAR2 ELISA Kit (Colorimetric)

Human PAR2 ELISA Kit (Colorimetric)

ELISA: Human PAR2 ELISA Kit (Colorimetric) [NBP3-06966] -

ELISA: Human PAR2 ELISA Kit (Colorimetric) [NBP3-06966] - Standard Curve Reference

Kit Contents for Human PAR2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PAR2

The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries.

Long Name

Protease-Activated Receptor 2

Alternate Names

CFIIRL1, F2RL1, GPCR11

Gene Symbol

F2RL1

Additional PAR2 Products

Product Documents for Human PAR2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PAR2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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