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Rat PAR2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06968

Novus Biologicals, part of Bio-Techne
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NBP3-06968

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

4.69 nIU/mL

Assay Range

7.81 - 500 nIU/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat PAR2 in samples. No significant cross-reactivity or interference between Rat PAR2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV > 5.55

Inter-Assay Precision (Precision between assays) %CV > 5.47

Recovery for Rat PAR2 ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Rat PAR2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat PAR2 ELISA Kit (Colorimetric)

Rat PAR2 ELISA Kit (Colorimetric)

ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] -

ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Standard Curve Reference

Kit Contents for Rat PAR2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PAR2

The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries.

Long Name

Protease-Activated Receptor 2

Alternate Names

CFIIRL1, F2RL1, GPCR11

Gene Symbol

F2RL1

Additional PAR2 Products

Product Documents for Rat PAR2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat PAR2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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