Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06970

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all PAR3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human PAR3/Thrombin Receptor-like 2 in samples. No significant cross-reactivity or interference between Human PAR3/Thrombin Receptor-like 2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.41

Inter-Assay Precision (Precision between assays) 5.31

Recovery for Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

Recovery

84-105 %

Linearity

Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

Scientific Data Images for Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

ELISA: Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric) [NBP3-06970] -

ELISA: Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric) [NBP3-06970] - Standard Curve Reference

Kit Contents for Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PAR3

PAR3, also known as F2RL2 and Coagulation Factor II (Thrombin) Receptor-like 2, is a member of the Protease-activated Receptor (PAR) family of seven transmembrane G protein-coupled receptors that are activated by proteolysis. It is 374 amino acids (aa) in length with a predicted molecular weight of 42.5 kDa. Human PAR3 shares 72% aa sequence identity with the mouse and rat orthologs. PAR3 is highly expressed in the megakaryocytes of the bone marrow. It is also expressed, but in lower levels, in mature megakaryocytes, vascular endothelial and smooth muscle cells, platelets, and in a variety of other tissues such as heart and small intestines. PAR3 plays an essential role in hemostasis and thrombosis. Thrombin, a coagulation protease that activates platelets, binds to and cleaves the N-terminal extracellular domain of PAR3 to generate a new N-terminus. This new N-terminus then functions as a tethered peptide ligand that binds to the receptor. Interestingly, it appears the PAR3 is unable to mediate signaling responses to Thrombin. Instead, it seems to act as a cofactor for two other PAR family members, PAR1 and PAR4.

Long Name

Protease-Activated Receptor 3

Alternate Names

F2RL2, Protease-activated receptor 3, Thrombin Receptor-Like 2

Gene Symbol

F2RL2

Additional PAR3 Products

Product Documents for Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PAR3/Thrombin Receptor-like 2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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