Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69890

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all PEDFR/PNPLA2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ATGL in samples. No significant cross-reactivity or interference between Rat ATGL and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.32%

Inter-Assay Precision (Precision between assays) CV% < 4.83%

Recovery for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

Recovery

86-109%

Linearity

Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

Scientific Data Images for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

ELISA: Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric) [NBP2-69890] -

ELISA: Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric) [NBP2-69890] - Standard Curve Reference

Kit Contents for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PEDFR/PNPLA2

Patatin-like phospholipase domain-containing protein 2 (PNPLA2), also known as adipose triglyceride lipase (ATGL), is a lipolytic enzyme required for mobilization of fatty acids from triglyceride stores in adipose tissue. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. However, ATGL has now been shown to catalyze the initial step of triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Thus, ATGL and HSL coordinate to catabolize stored triglycerides in adipose tissue of mammals.

Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.

ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Gene Symbol

PNPLA2

Additional PEDFR/PNPLA2 Products

Product Documents for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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