Human PER1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39300

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.114 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

View all PER1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human PER1 ELISA Kit (Colorimetric)

Human PER1 ELISA Kit (Colorimetric)

ELISA: Human PER1 ELISA Kit (Colorimetric) [NBP3-39300]

ELISA: Human PER1 ELISA Kit (Colorimetric) [NBP3-39300] - Standard Curve Reference

Kit Contents for Human PER1 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PER1

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Long Name

Period Circadian Protein 1

Alternate Names

PERIOD1, RIGUI

Gene Symbol

PER1

Additional PER1 Products

Product Documents for Human PER1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PER1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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