Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39411

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39411

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.57 ng/mL (example only; lot dependent)

Assay Range

1.56 - 100 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Plectin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39411]

ELISA: Human Plectin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39411] - Standard Curve Reference

Kit Contents for Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Plectin

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Alternate Names

EBS1, HD1, Hemidesmosomal protein 1, LGMD2Q, PCNplectin 1, intermediate filament binding protein, 500kD, PLEC1b, PLEC1EBSO, plectin, plectin 1, intermediate filament binding protein 500kDa, plectin-1, PLTNepidermolysis bullosa simplex 1 (Ogna)

Gene Symbol

PLEC

Additional Plectin Products

Product Documents for Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Plectin - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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