Human Podocin/NPHS2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82136

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Podocin/NPHS2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Podocin/NPHS2 in samples. No significant cross-reactivity or interference between Human Podocin/NPHS2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Human Podocin/NPHS2 ELISA Kit (Colorimetric)

Recovery

average = 100% for serum; average = 93% for EDTA plasma; average = 101% for cell culture media

Linearity

Human Podocin/NPHS2 ELISA Kit (Colorimetric)

Scientific Data Images for Human Podocin/NPHS2 ELISA Kit (Colorimetric)

Human Podocin/NPHS2 ELISA Kit (Colorimetric)

ELISA: Human Podocin/NPHS2 ELISA Kit (Colorimetric) [NBP2-82136] -

ELISA: Human Podocin/NPHS2 ELISA Kit (Colorimetric) [NBP2-82136] - Standard Curve Reference

Kit Contents for Human Podocin/NPHS2 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Podocin/NPHS2

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Alternate Names

Nephrosis 2, NPHS2, PDCN, SRN1

Gene Symbol

NPHS2

Additional Podocin/NPHS2 Products

Product Documents for Human Podocin/NPHS2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Podocin/NPHS2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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