Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # P1240
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Conjugate
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 27 | 27 | 27 |
Mean (pg/mL) | 89.8 | 362 | 819 | 88.8 | 361 | 795 |
Standard Deviation | 7.3 | 17.7 | 28.0 | 9.2 | 22.9 | 46.1 |
CV% | 8.1 | 4.9 | 3.4 | 10.4 | 6.3 | 5.8 |
EDTA Plasma, Heparin Plasma, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 25 | 25 | 25 |
Mean (pg/mL) | 115 | 447 | 991 | 110 | 454 | 995 |
Standard Deviation | 7.4 | 22.7 | 33.0 | 10.1 | 33.4 | 64.3 |
CV% | 6.4 | 5.1 | 3.3 | 9.2 | 7.4 | 6.5 |
Recovery for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
The recovery of porcine IL-12/IL-23 p40 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=4) | 104 | 97-113 |
EDTA Plasma (n=4) | 92 | 84-99 |
Heparin Plasma (n=5) | 94 | 85-105 |
Serum (n=5) | 99 | 88-109 |
Linearity
To assess the linearity of the assay, samples spiked with or containing porcine IL-12/IL-23 p40 in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
Porcine IL-12/IL-23 p40 ELISA Calibrator Diluent RD5Y Standard Curve
Porcine IL-12/IL-23 p40 ELISA Calibrator Diluent RD6-3 Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-12/IL-23 p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-12/IL-23 p40 Products
Product Documents for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
Product Specific Notices for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.