What is the species reactivity of the Prostaglandin E2 Parameter Assay Kit?

The Parameter PGE2 Immunoassay is a competitive enzyme immunoassay designed to measure PGE2 level in various sample types, and the kit can be used for multiple species.

How should the indomethacin be prepared, prior to being added to the serum or plasma collection tubes?

First, dissolve indomethacin (Tocris cat # 1708) in ethanol to 10 mg/mL. Then, dilute indomethacin solution 1:50 in 1X PBS (to 0.2 mg/mL). Finally, inject 0.2 mg/mL indomethacin solution into vacutainer tube immediately following blood draw, to a final concentration of 10 μg/mL.

Prostaglandin E2 Parameter Assay Kit Best Seller

Name: Prostaglandin E2 Parameter Assay Kit
Brand: R&D Systems
SKU: KGE004B
Rating: 3 (12 reviews)

R&D Systems, part of Bio-Techne | Catalog # KGE004B

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (51 µL), Serum (51 µL), EDTA Plasma (51 µL), Heparin Plasma (51 µL), Urine (51 µL)

Sensitivity

41.4 pg/mL

Assay Range

39-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

View all Prostaglandin E2/PGE2 ELISA Kits »

Product Summary for Prostaglandin E2 Parameter Assay Kit

The Parameter PGE₂ Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE₂ in cell culture supernates, serum, plasma, and urine.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Reactivity

Multi-Species

Specificity

Measures PGE₂ levels in various samples

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	234	895	1456	253	835	1331
Standard Deviation	21	45	89	33	82	120
CV%	9.0	5.0	6.1	12.9	9.9	9.0

Recovery for Prostaglandin E2 Parameter Assay Kit

The recovery of PGE₂ spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	84-106
EDTA Plasma (n=4)	93	82-108
Heparin Plasma (n=4)	94	81-111
Serum (n=4)	97	86-114
Urine (n=4)	99	81-111

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PGE<SUB>2</SUB> were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Prostaglandin E2 Parameter Assay Kit

Multi-species Prostaglandin E2/PGE2 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Prostaglandin E2/PGE2

Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

Alternate Names

Dinoprostone

Additional Prostaglandin E2/PGE2 Products

Product Documents for Prostaglandin E2 Parameter Assay Kit

Product Datasheets

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COA

SDS

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Product Specific Notices for Prostaglandin E2 Parameter Assay Kit

For research use only

Citations
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.