Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80325

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Lysate (100 ul)

Sensitivity

42.02 pg/ml

Assay Range

125-8000 pg/ml (example only; lot dependent)

View all Protein Disulfide Isomerase/P4HB ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human Protein Disulfide Isomerase/P4HB.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.2

Inter-Assay Precision (Precision between assays) CV% < 5.3 %

Recovery for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Recovery

78-82%

Scientific Data Images for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325]

ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325]

ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325] - Example standard curve for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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