Rat IL-6 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # R6000B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
Sensitivity
36 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Rat IL-6 Quantikine ELISA Kit
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, rat serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat IL-6 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-6.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat IL-6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 27 | 28 |
| Mean (pg/mL) | 120 | 256 | 1028 | 134 | 268 | 987 |
| Standard Deviation | 10.6 | 22.4 | 46.2 | 13.4 | 21.7 | 69.2 |
| CV% | 8.8 | 8.8 | 4.5 | 10.0 | 8.1 | 7.0 |
Recovery for Rat IL-6 Quantikine ELISA Kit
The recovery of rat IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 103 | 85-118 |
| EDTA Plasma (n=5) | 96 | 81-111 |
| Serum (n=5) | 97 | 91-105 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat IL-6 Quantikine ELISA Kit
Rat IL-6 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a
component required for signal transduction (4). The rat, mouse and human IL-6 cDNAs have
been cloned (6-9). Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide
with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature
protein. At the protein sequence level, there is approximately 39% identity between rat and
human, and 87% identity between mouse and rat IL-6 (6). Although human and mouse IL-6 are
equally active on mouse cells, mouse IL-6 is not active on human cells (9).
The high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two
membrane glycoproteins: an 80 kDa low-affinity IL-6-binding receptor (IL-6 R) and a 130 kDa
signal-transducing protein (gp130) that lacks IL-6 binding ability (4). The binding of IL-6 to
IL-6 R recruits gp130 to form a trimeric complex that dimerizes to form a hexameric complex,
which transduces IL-6 signal (4). Soluble forms of both IL-6 R and gp130 have been detected in
blood (11, 12). Soluble IL-6 R is capable of binding IL-6 and inducing homodimerization of
membrane gp130 and subsequent signal transduction.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
Additional IL-6 Products
Product Documents for Rat IL-6 Quantikine ELISA Kit
Product Specific Notices for Rat IL-6 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.